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A cDNA library was constructed from early stage ovaries of diploid apomictic H. piloselloides D2 undergoing the early events of apomictic reproduction (Stage 2,3,4; Koltunow et al 1998; Tucker et al., 2001). Genes that were predominantly ovary-specific and expressed at a low level were identified by cold plaque screening, using leaf and ovary cDNAs (Hodge et al., 1992).
Nucleotide
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