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NICHD_XGC_Tad1

Identifiers
BioSample: SAMN00153969; EST: LIBEST_012612
Organism
Xenopus laevis (African clawed frog)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus
Attributes
development stagemetamorphosis stage 53
tissueDeveloping Tadpole
vectorpDNR-LIB
v_typeplasmid (chloramphenoicol resistant, grown at 30C)
re_1Sfi
re_2Sfi
Description

5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.6 kb (range 0.9-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

Submission
National Cancer Institute / NIH, Daniela S. Gerhard, Ph.D.; 2003-01-30
Accession:
SAMN00153969
ID:
153969

Nucleotide

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