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Total RNA was extracted from the control group and the treated group of Dendrobium nobile leaf and stem induced by AR-18, one mycorrhizal fungus, respectively. DDRT-PCR, reverse Northern blot and Southern blot analysis were used to isolate and identify genes whose transcription were altered in cultured D. nobile plants that were treated with AR-18. The differentially displayed cDNAs were cloned directionally into the pBluescript SK(+/-) plasmid.
Nucleotide
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