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ERR7746411_bin.310

Identifiers
BioSample: SAMEA13475686; SRA: ERS11077738
Organism
uncultured Methanosphaera sp.
cellular organisms; Archaea; Methanobacteriati; Methanobacteriota; Methanomada group; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanosphaera; environmental samples
Attributes
collection date2013-09-07
broad-scale environmental contextterrestrial biome
local-scale environmental contexthuman-associated habitat
environmental mediumfeces
geographic locationTanzania
investigation typemetagenome-assembled genome
isolation sourcehuman feces
project nameMetagenome sequencing of Hadza gut microbiome
reference for biomaterialhttps://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103
sample collection device or methodhttps://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103
sample nameREFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_PheChl_Fiber-Hadza-Nepal_A_2_1112.310
16S rRNAs recovered0
16S recoveredFALSE
16S recovery softwarebarrnap;0.9;--kingdom arc
23S rRNAs recovered0
5S rRNAs recovered0
CheckM strain heterogeneity0
ENA first public2022-04-01
ENA last update2022-04-01
ENA-CHECKLISTERC000011
External IdSAMEA13475686
GTDB r202 classificationd__Archaea;p__Methanobacteriota;c__Methanobacteria;o__Methanobacteriales;f__Methanobacteriaceae;g__Methanosphaera;s__Methanosphaera stadtmanae
INSDC center aliasStanford University School of Medicine
INSDC center namestanford university school of medicine
INSDC first public2022-04-01T08:24:49Z
INSDC last update2022-04-01T08:24:49Z
INSDC statuspublic
MAG coverage depth19.27
MAG coverage softwareReads (ERR7746411) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4568013) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.
Submitter IdREFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_PheChl_Fiber-Hadza-Nepal_A_2_1112.310
assembly qualityMany fragments with little to no review of assembly other than reporting of standard assembly statistics.
assembly softwaremetaSPAdes;3.13.1;-k 21,33,55,77 --merged BBMerge.fq.gz -1 R1.fq.gz -2 R2.fq.gz
binning parametersReads from ERR7746411 were assembled into contigs (ERZ4568013). The following read sets from this study were the most similar to ERR7746411 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7746411,ERR7745759,ERR7745743,ERR7747255,ERR7738557,ERR7745408,ERR7738626,ERR7747394,ERR7745823,ERR7746349. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4568013. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.
binning softwareMetaBAT2 v2.15; MAGpurify v2.1.0
completeness approachCheckM marker lineage: p__Euryarchaeota (UID3)
completeness score97.6
completeness softwareCheckM;1.1.2;lineage_wf
contamination score0
contamination screening inputreads
contamination screening parametersAll reads in ERR7746411 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).
geographic location (elevation)1287
geographic location (latitude)-3.55
geographic location (longitude)-34.58
inter-study species representativeGUT_GENOME143494
intra-study species representativeERR7738600_bin.106
metagenomic sourcehuman gut metagenome
nucleic acid extractionhttps://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103
number of standard tRNAs extracted39
reassembly post binningNo
sample derived fromERS2585536
sequencing methodIllumina NovaSeq 6000
tRNA extraction softwaretRNAscan-SE;2.0.9;-A
taxonomic classificationGTDB-TK v1.7.0, r202 database, default parameters
taxonomic identity markermulti-marker approach
unique standard tRNAs extracted20
Description

This sample represents an uncultured Methanosphaera sp. MAG assembled and binned from ERR7746411.

BioProject
PRJEB49206 Ultra-deep Sequencing of Hadza Hunter-Gatherers Recovers Vanishing Microbes
Retrieve all samples from this project

Submission
EBI; 2022-04-02
Accession:
SAMEA13475686
ID:
27197197

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