GQ004: Non-lignified secondary xylem from mature t... - BioSample - NCBI

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GQ004: Non-lignified secondary xylem from mature trees

Identifiers: BioSample: SAMN00154061; EST: LIBEST_015130

Organism: Picea glauca (white spruce)
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Acrogymnospermae; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Picea

Attributes

strain	Two trees of provenance 5333 and one from 5206
sex	Hermaphrodite
tissue	Non-lignified differentiating secondary xylem from normal vertical trees
development stage	Non-lignified secondary xylem from trees harvested 2.5 hours, 6 hours and 11 hours after day break
lab host	E. coli DH10B cells
tissue	Stem from ground to lower part of live crown, on 33 year old tree
vector	pBluescript II SK (+) XR
v_type	Plasmid
re_1	Eco-RI
re_2	Xho-I

Description: Tissue was harvested in mid-June, during formation of early wood. cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

Submission: Universite Laval, John MacKay; 2004-01-08

Accession:: SAMN00154061
ID:: 154061

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GQ004: Non-lignified secondary xylem from mature trees
GQ004: Non-lignified secondary xylem from mature trees

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