CCGC Panicum virgatum early floral buds + reproduc... - BioSample

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Result Filters

Organism: Panicum virgatum (switchgrass)
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; PACMAD clade; Panicoideae; Panicodae; Paniceae; Panicinae; Panicum; Panicum sect. Hiantes

Attributes

cultivar	pool of Kanlow, Summer, Shawnee
lab host	DH10B T1-resistant
tissue	early floral buds + reproductive tissue
vector	pCMV-SPORT6
re_1	SalI
re_2	NotI

Description: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).