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UI-E-CI1

Identifiers
BioSample: SAMN00170180; EST: LIBEST_010285
Organism
Homo sapiens (human)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo
Attributes
tissueRPE and Choroid
development stageadult
lab hostDH10B (Life Technologies) (T1 phage resistant)
tissueeye
vectorpT7T3-Pac (Pharmacia) with a modified polylinker
v_typePlasmid
re_1EcoR I
re_2Not I
Description

UI-E-CI1 is a normalized cDNA library containing the following tissue(s): RPE and Choroid. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is ACCTA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).

Submission
Children's Memorial Research Center, Soares, MB; 2002-02-27
Accession:
SAMN00170180
ID:
170180

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