BioSample

In Vivo Conditions: Stressed by starvation. The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library was created by the Daphnia Genomics Consortium and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.