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NICHD_XGC_tropPanc1

Identifiers
BioSample: SAMN00166631; EST: LIBEST_020931
Organism
Xenopus tropicalis (tropical clawed frog)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana
Attributes
strainNigerian
tissuePancreas
development stageAdult
lab hostElectroMAX DH10B T1 Phage Resistant cells
vectorpCS107
re_1SalI
re_2NotI
Description

This library was made from dT primed cDNA and cloned into pCS107 vector. The work was done at DOE Joint Genome Institute. Poly A RNA were primed with 5' GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT 3'. CDNA were ligated to SalI adapter (5' TCGACCCACGCGTCCG and 5'CGGACGCGTGGG), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into NotI and SalI digested pCS107 vector.

Submission
DOE Joint Genome Institute, Lindquist,E.A., Richardson,P.; 2007-03-12
Accession:
SAMN00166631
ID:
166631

Nucleotide

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