NICHD_XGC_tropSp1 - BioSample - NCBI

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NICHD_XGC_tropSp1

Identifiers: BioSample: SAMN00166630; EST: LIBEST_020912

Organism: Xenopus tropicalis (tropical clawed frog)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana

Attributes

tissue	Spleen
development stage	Adult
lab host	ElectroMAX DH10B T1 Phage Resistent cells
vector	pCS107
re_1	SalI
re_2	NotI

Description: This library was made from dT primed cDNA and cloned into pCS107 vector. The work was done at DOE Joint Genome Institute. Poly A RNA were primed with 5' GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT 3'. CDNA were ligated to SalI adapter (5' TCGACCCACGCGTCCG and 5'CGGACGCGTGGG), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into NotI and SalI digested pCS107 vector.

Submission: DOE Joint Genome Institute, Lindquist,E.A., Richardson,P.; 2007-03-06

Accession:: SAMN00166630
ID:: 166630

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BioSample links for Nucleotide (Select 126607239) (1)
BioSample
NICHD_XGC_tropSp1
NICHD_XGC_tropSp1

biosample
CBSS4714.fwd NICHD_XGC_tropSp1 Xenopus tropicalis cDNA clone IMAGE:8897045 5', m...
CBSS4714.fwd NICHD_XGC_tropSp1 Xenopus tropicalis cDNA clone IMAGE:8897045 5', mRNA sequence
gi|126607239|gnl|dbEST|45116819|gb| 460.1|

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