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ROMEO2015.030.RNA_2015-09-21_Yuk_C_summer_river_water

Identifiers
BioSample: SAMN15506180; Sample name: ROMEO2015.030.RNA; SRA: SRS6986063
Organism
freshwater metagenome
unclassified entries; unclassified sequences; metagenomes; ecological metagenomes
Package
Metagenome or environmental; version 1.0
Attributes
isolation sourceriver water
collection date2015-09-21
geographic locationUSA: Yukon River at Pilot Station, AK
latitude and longitude61.933690 N 162.882940 W
sample collection device or methodCollected into a 10L carboy dipped below the surface near the center of the river channel
sample material processingMaterial pre-filtered with a 3.0 micron pore-size, 142mm diameter polycarbonate filter, and collected on a 0.22 micron pore-size Sterivex filter (Millipore, Burlington, MA). Artificial transcript sequences (https://www.addgene.org; products MTST5 and MTST6) were added (0.65 ng of each) prior to nucleic acid extraction for quantification analyses, as per Satinsky et al. 2013 (https://doi.org/10.1016/B978-0-12-407863-5.00012-5). Following RNA extraction using the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, Middleton, WI), mRNA was enriched using Ribo-Zero microbial rRNA removal kit (Illumina, Inc.)
sample size1.3 L
isolateYuk_C_summer
replicatereplicate C
Description

USGS 15565447 Yukon River at Pilot Station, AK

BioProject
PRJNA645466
Retrieve all samples from this project

Submission
Oregon State University, Byron Crump; 2020-07-10
Accession:
SAMN15506180
ID:
15506180

BioProject SRA

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