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ROMEO2015.014.RNA_2015-05-27_StLaw_B_spring_river_water

Identifiers
BioSample: SAMN15506164; Sample name: ROMEO2015.014.RNA; SRA: SRS6986046
Organism
freshwater metagenome
unclassified entries; unclassified sequences; metagenomes; ecological metagenomes
Package
Metagenome or environmental; version 1.0
Attributes
isolation sourceriver water
collection date2015-05-27
geographic locationUSA: St. Lawrence River at Cornwall, Ontario, near Massena, NY
latitude and longitude45.006336 N 74.795107 W
sample collection device or methodCollected into a 10L carboy filled with water passing through the Moses-Saunders Power Dam, consistent with water quality samples collected by the USGS on that day
sample material processingMaterial pre-filtered with a 3.0 micron pore-size, 142mm diameter polycarbonate filter, and collected on a 0.22 micron pore-size Sterivex filter (Millipore, Burlington, MA). Artificial transcript sequences (https://www.addgene.org; products MTST5 and MTST6) were added (0.65 ng of each) prior to nucleic acid extraction for quantification analyses, as per Satinsky et al. 2013 (https://doi.org/10.1016/B978-0-12-407863-5.00012-5). Following RNA extraction using the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, Middleton, WI), mRNA was enriched using Ribo-Zero microbial rRNA removal kit (Illumina, Inc.)
sample size5.61 L
isolateStLaw_B_spring
replicatereplicate B
Description

USGS 04264331 St. Lawrence River at Cornwall, Ontario, near Massena, NY

BioProject
PRJNA645466
Retrieve all samples from this project

Submission
Oregon State University, Byron Crump; 2020-07-10
Accession:
SAMN15506164
ID:
15506164

BioProject SRA

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