| cultivar | sp.B |
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| biomaterial provider | Adam Mickiewicz University, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Department of Gene Expression |
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| tissue | mixture of male and female thalli with sporophyte included |
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| age | various |
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| collected by | Halina Pietrykowska, Pawel Piszczalka, Izabela Sierocka |
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| collection date | 2011 |
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| development stage | For in vitro plants: vegetative haploid thalli; for natural habitat plants: vegetative haploid thalli producing sex organs with sporophytes included. |
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| geographic location | Poland: Poznan, Kopanina site |
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| growth protocol | Plants are grown on 1/2 Gamborg medium (5.5-6.0 pH) solidified with agar. For auxenic cultures, constant, white fluorescent light (~1000 lux) is used and temperature of 21-24 oC is maintained. |
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| height or length | ~1cm wide, flat thalli, grows in patches and clusters ranging from few centimeters up to over 1m in diameter |
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| isolation source | Natural habitat plants: semi-wet meadows, near abandoned and flooded clay-pits. Plants usually spotted on the edge zone between flooded tall canes and drier, higher areas overgrown with grass and low bushes. |
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| latitude and longitude | 52.36 N 16.85 E |
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| sample type | whole plant without rhizoids |
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| specimen voucher | Adam Mickiewicz Herbarium, 40228 POZW |
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| treatment | For natural habitat plants: soil, other plant species and animal contaminants were removed. For auxenic plants: agar leftovers were discarded. Both types of thalli had their rhizoids removed before isolation. |
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