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T47D_E2_MPA_2

Identifiers
BioSample: SAMN02851193; Sample name: T47D_E2_MPA_2
Organism
Homo sapiens (human)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo
Package
Human; version 1.0
Attributes
isolateT47-D
ageP45
biomaterial providerGrant Buchanan, Cancer Biology Group, The University of Adelaide, School of Medicine, Basil Hetzel Institute for Translational Health Research, 28 Woodville Rd, Woodville South SA 5011, Australia
sexfemale
tissueBreast
cell lineT47_D
cell typeBreast carcinoma
culture collectionATCC:HTB-133
sample typeRNA
treatmentHormone starved, 16h 10nM medroxyprogesterone acetate and 16h 10nM 17beta-estradiol
replicate number2
Description

Cells plated at 300000 cells per well in phenol red free RPMI1640 supplemented with 10% hormone stripped FBS for 48h before treatment. RNA exctracted with Qiagen Rneasy kit with Dnase digestion on column

BioProject
PRJNA252531 Homo sapiens
Retrieve all samples from this project

Submission
The University of Adelaide, Eleanor Need; 2014-06-11
Accession:
SAMN02851193
ID:
2851193

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