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Seawater from the long-term ecological research station Helgoland Roads (54°11’03”N, 7°54’00”E) was sampled on the 28th April, 2016 and serially diluted with artificial seawater. An inoculum of 2.58 nl with statistically 3 cells grew in an oligotrophic HaHa medium with the addition of vitamins in the dark at 12°C. After several transfers and another dilution to extinction series, purity controls confirmed that the culture contained a pure strain. It was maintained by transfers every three months. Growth in HaHa100V medium yielded a turbid, orange-coloured culture and provided biomass for DNA extraction.
BioProject SRA Nucleotide
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