| depth | 391 |
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| broad-scale environmental context | Polar Biome |
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| local-scale environmental context | [MES] marine water layer (ENVO:01000295) within the mesopelagic zone (ENVO:00000213) |
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| environmental medium | water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063) |
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| environmental package | water |
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| project name | Tara Oceans Expedition (2009-2013) |
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| sample name | TARA_B110000438 |
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| size-fraction lower threshold | 0.22 |
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| size-fraction upper threshold | 3 |
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| temperature | 4.791395 |
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| Chlorophyll Sensor | -0.0036 |
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| Citation | Tara Oceans Consortium, Coordinators & Tara Oceans Expedition, Participants. Registry of all samples from the Tara Oceans Expedition (2009-2013). PANGAEA. doi:10.1594/PANGAEA.842197 (2015) (https://dx.doi.org/10.1594/PANGAEA.842197) |
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| ENA first public | 2019-10-15 |
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| ENA last update | 2016-08-25 |
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| ENA-CHECKLIST | ERC000030 |
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| Event Date/Time End | 2013-10-27T21:38:00 |
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| External Id | SAMEA4398413 |
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| INSDC center alias | GSC |
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| INSDC center name | Genoscope |
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| INSDC first public | 2019-10-15T04:04:43Z |
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| INSDC last update | 2016-08-25T12:24:58Z |
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| INSDC status | public |
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| Latitude End | 61.5128 |
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| Longitude End | -55.9886 |
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| Salinity Sensor | 34.939595 |
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| Submitter Id | TARA_B110000438 |
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| environment (feature) further details | discrete depth around 400 m, within the mesopelagic zone (ENVO:00000213) within the aphotic zone (ENVO:00000210) |
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| event date/time start | 2013-10-27T16:30:00 |
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| event label | TARA_20131027T1630Z_210_Combined-EVENTS_CAST |
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| further details | http://store.pangaea.de/Projects/TARA-OCEANS/Logsheets_Wetlab/TARA_20131027Z_210_WETLAB_BACT.pdf |
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| last update date | 2016-04-23Z |
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| latitude start | 61.5754 |
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| longitude start | -55.9846 |
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| marine region | [AO] Arctic Ocean (MRGID:1906) |
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| nitrate sensor | 16.017641 |
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| oxygen sensor | 271.8744 |
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| protocol description | In the size fraction W0.22-3 micrometres, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 0.22-micrometre-pore-size filter was packaged in a sterile Falcon tube (50 mL), treated with the addition of 10 mL of RNA-Later, labelled with a barcode identification sticker and stored on board in a freezer at -20 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump (Masterflex, EW-77410-10) was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. A total of 2 replicates were prepared and sent for later analysis to the Institute of Marine Science (CSIC), Barcelona, Spain. |
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| protocol label | SEQ-(100L)_W0.22-3 |
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| sample collection device | Rosette Vertical Sampling System [RVSS] |
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| sample material label | TARA_210_MES_0.22-3 |
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| sample status | This version can be used to provide data discovery services |
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| sampling campaign | TARA_20131020Z |
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| sampling platform | SV Tara |
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| sampling station | TARA_210 |
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