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Metagenome-assembled genome: SRR18243793_bin.40_metawrap_v1.3_MAG

Identifiers
BioSample: SAMEA112250254; SRA: ERS14361117
Organism
uncultured Candidatus Saccharibacteria bacterium
cellular organisms; Bacteria; Bacteria incertae sedis; Bacteria candidate phyla; Candidatus Saccharibacteria; environmental samples
Attributes
broad-scale environmental contextmouse digestive system
collection date2016-01-21
environmental mediumfeces
geographic locationGermany
investigation typemetagenome-assembled genome
isolation sourcemouse gut metagenome
project nameHypertension (HTN) can lead to heart and kidney damage. The gut microbiota has been linked to HTN, although it is difficult to estimate its significance due to the variety of other features known to influence HTN. In the present study, we used germ-free (GF) and colonized (COL) littermate mice to quantify the impact of microbial colonization on organ damage in HTN.</p><p>Four-week-old male GF C57BL/6J littermates were randomized to remain GF or receive microbial colonization. HTN was induced by subcutaneous infusion with angiotensin (Ang) II (1.44mg/kg/d) and 1% NaCl in the drinking water; sham-treated mice served as control. Renal damage was exacerbated in GF mice, whereas cardiac damage was more comparable between COL and GF, suggesting that the kidney is more sensitive to microbial influence. Multivariate analysis revealed a larger effect of HTN in GF mice. Serum metabolomics demonstrated that the colonization status influences circulating metabolites relevant to HTN. Importantly, GF mice were deficient in anti-inflammatory fecal short-chain fatty acids (SCFA). Flow cytometry showed that the microbiome has an impact on the induction of anti-hypertensive myeloid-derived suppressor cells and pro-inflammatory Th17 cells in HTN. In vitro inducibility of Th17 cells was significantly higher for cells isolated from GF than conventionally raised mice.</p><p>Microbial colonization status of mice had potent effects on their phenotypic response to a hypertensive stimulus, and the kidney is a highly microbiota-susceptible target organ in HTN. The magnitude of the pathogenic response in GF mice underscores the role of the microbiome in mediating inflammation in HTN.
sample nameSRR18243793_bin.40_metawrap_v1.3_MAG
ENA-CHECKLISTERC000047
ENA-FIRST-PUBLIC2022-12-17
ENA-LAST-UPDATE2022-12-17
External IdSAMEA112250254
INSDC center aliasEMG
INSDC center nameEMG
INSDC first public2022-12-17T00:47:18Z
INSDC last update2022-12-17T00:47:18Z
INSDC statuspublic
Submitter IdSRR18243793_bin.40_metawrap_v1.3_MAG
assembly qualityMany fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly softwarenot provided
binning parametersMaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.
binning softwaremetawrap v1.3
broker nameEMG broker account, EMBL-EBI
completeness score62.03
completeness softwareCheckM
contamination score0.85
geographic location (latitude)52.62686
geographic location (longitude)13.507567
local environmental contextdigestive tube
metagenomic sourcemouse gut metagenome
sample derived fromSAMN26378643
sequencing methodIllumina NovaSeq 6000
taxonomic classificationThe taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Candidatus Saccharibacteria;c__;o__;f__;g__;s__
taxonomic identity markermulti-marker approach
Description

This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR18243793 of study SRP362674.

BioProject
PRJEB58022
Retrieve all samples from this project

Submission
EBI; 2022-12-18
Accession:
SAMEA112250254
ID:
32300666

BioProject Nucleotide

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