| depth | 5 |
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| broad-scale environmental context | Polar Biome |
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| local-scale environmental context | [SRF] surface water layer (ENVO:00010504) |
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| environmental medium | water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063) |
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| environmental package | water |
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| project name | Tara Oceans Expedition (2009-2013) |
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| sample name | TARA_B110000259 |
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| size-fraction lower threshold | 0.22 |
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| size-fraction upper threshold | 3 |
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| temperature | 0.030106 |
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| Chlorophyll Sensor | 0.474975 |
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| Citation | Tara Oceans Consortium, Coordinators & Tara Oceans Expedition, Participants. Registry of all samples from the Tara Oceans Expedition (2009-2013). PANGAEA. doi:10.1594/PANGAEA.842197 (2015) (https://dx.doi.org/10.1594/PANGAEA.842197) |
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| ENA first public | 2019-10-15 |
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| ENA last update | 2016-08-25 |
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| ENA-CHECKLIST | ERC000030 |
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| Event Date/Time End | 2013-08-27T03:48:00 |
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| External Id | SAMEA4397649 |
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| INSDC center alias | GSC |
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| INSDC center name | Genoscope |
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| INSDC first public | 2019-10-15T04:04:43Z |
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| INSDC last update | 2016-08-25T12:05:20Z |
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| INSDC status | public |
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| Latitude End | 77.9295 |
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| Longitude End | 116.9938 |
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| Salinity Sensor | 28.576956 |
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| Submitter Id | TARA_B110000259 |
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| environment (feature) further details | discrete depth within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209), <50 m from a strong peak in chlorophyll fluorescence |
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| event date/time start | 2013-08-27T01:53:00 |
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| event label | TARA_20130827T0153Z_189_EVENT_PUMP |
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| further details | http://store.pangaea.de/Projects/TARA-OCEANS/Logsheets_Wetlab/TARA_20130827Z_189_WETLAB_BACT.pdf |
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| last update date | 2016-04-23Z |
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| latitude start | 77.9028 |
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| longitude start | 117.1545 |
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| marine region | [AO] Arctic Ocean (MRGID:1906) |
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| nitrate sensor | 99999 |
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| oxygen sensor | 364.342188 |
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| protocol description | In the size fraction W0.22-3 micrometres, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 0.22-micrometre-pore-size filter was packaged in a sterile Falcon tube (50 mL), treated with the addition of 10 mL of RNA-Later, labelled with a barcode identification sticker and stored on board in a freezer at -20 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump (Masterflex, EW-77410-10) was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. A total of 2 replicates were prepared and sent for later analysis to the Institute of Marine Science (CSIC), Barcelona, Spain. |
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| protocol label | SEQ-(100L)_W0.22-3 |
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| sample collection device | high volume peristaltic pump [HVP-PUMP] |
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| sample material label | TARA_189_SRF_0.22-3 |
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| sample status | This version can be used to provide data discovery services |
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| sampling campaign | TARA_20130801Z |
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| sampling platform | SV Tara |
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| sampling station | TARA_189 |
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