LUGEMM14 7-63

Organism: Mus musculus (house mouse)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus

Attributes

age	D20
collection date	not collected
genotype	wild type genotype
geographic location	not collected
isolate	not applicable
tissue	lung
sample name	E-MTAB-13704:LUGEMM14 7-63
ENA first public	2024-01-29
ENA-CHECKLIST	ERC000011
INSDC center name	Oncology Data Science Oncology R&D
INSDC status	public
Submitter Id	E-MTAB-13704:LUGEMM14 7-63
broker name	ArrayExpress
common name	house mouse
sampling site	tumour
scientific_name	Mus musculus
tumours_numbers	12

Description: Protocols: Samples were collected from GEMMs lung lesions and assessed by MRI quantification of combined lung tumour lesions per animal before & after treatment RNA was extracted using the RNeasy Mini Kit (Qiagen; #74104). RNA concentration was determined by Qubit Flex Fluorometer (Invitrogen), RNA purity was determined using a NanoDrop Eight (Thermo Scientific) and RNA integrity was measured using a 4200 Tapestation (Agilent) Libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs; E7760L) or NEBNext® Ultra™ II RNA Library Prep Kit (New England BioLabs; E7770L) as per manufactuer’s guidelines. Ribosomal RNA was removed using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England BioLabs; E7490L) .

BioProject: PRJEB71814 RNAseq analysis of tumours from GEMMS animals to determine the effect of different aPDL1 combinations on lung tumours in situ
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