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Protocols: Leaf samples were taken on 12/09/2013, towards the end of the experiment, and flash-frozen in liquid nitrogen. All genotypes were exposed to three treatment conditions (“control”, “drought” & “well-watered”. Each treatment condition; control, drought, and well-water was repeated in each of four blocks placed in a green house in a randomized block design. Total RNA was extracted using the RNeasy plant Mini kit from Qiagen according to the manufacturer’s instruction, including an on-column digest of residual genomic DNA The total RNA was converted into mRNA sequencing libraries using the Illumina TruSeq RNA Sample Preparation Kit (V2) according to the manufacturer’s instructions.
BioProject SRA
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