BioProject

We report a robust protocol to effectively maintain high RNA quality for immuno-fluorescence-based laser capture microdissection (LCM), enabling finely resolved spatial transcriptome acquisition with only tens of cells from snap-frozen or RNAlater-treated tissues. More...

We report a robust protocol to effectively maintain high RNA quality for immuno-fluorescence-based laser capture microdissection (LCM), enabling finely resolved spatial transcriptome acquisition with only tens of cells from snap-frozen or RNAlater-treated tissues. With the repertoire of readily-available antibodies, this method fulfils the potential of LCM to determine native transcriptomic landscapes, as exemplified here with the characterization of mouse lacteal tip and tube cells. Less...