BioProject

We report gene expression data for strains that overexpress the transcriptional repressors Yox1 or Yhp1 from a synthetic, estradiol-inducible promoter. More...

We report gene expression data for strains that overexpress the transcriptional repressors Yox1 or Yhp1 from a synthetic, estradiol-inducible promoter. Gene expression was also measured in strains that overexpress unphosphorylatable alleles of either Yox1 or Yhp1, to determine whether blocking phosphorylation of these proteins by Cdk1 changes the spectrum of regulated genes. Finally, gene expression was measured in corresponding control strains in which Yox1/Yhp1 proteins were expressed from their endogenous promoters. These data demontsrate that Yox1 is a stronger repressor than Yhp1, although they regulate the same genes. In addition, phosphodeficient alleles of each TF regulate the same genes as the wild type proteins. Overall design: Gene expression was measured in asynchronous cells expressing wild type Yox1/Yhp1 proteins (YOX1, YHP1) or Yox1/Yhp1 proteins mutations in Cdk1 phosphoryation sites (yhp1_13A or yox1_9A). RNA was collected before estradiol addition (0) and 20 minutes (20) and 60 minutes (60) after the addition of estradiol. Matched samples were collected from controls strains (ctrl) expressing the same Yox1/Yhp1 proteins from their endogenous promoters. Three biological replicates were performed (rep1-rep3). Less...