Lhx8 is a member of the LIM-homeobox transcription factor family and preferentially expressed in oocytes and germ cells within the mouse ovary. We discovered that Lhx8 knockout females lose oocytes within 7 days after birth. At the time of birth, histological examination shows that Lhx8 deficient (Lhx8(-/-)) ovaries are grossly similar to the newborn wild type ovaries. Lhx8(-/-) ovaries fail to maintain the primordial follicles and the transition from primordial to growing follicles does not occur. Lhx8(-/-) ovaries misexpress oocyte-specific genes such as Gdf9, Pou5f1, and Nobox. Very rapid loss of oocytes may partly be due to drastic the down-regulation of Kit and Kitl in Lhx8(-/-) ovaries. We compared Lhx8(-/-) and wild-type ovaries using Affymetrix 430 2.
More...Lhx8 is a member of the LIM-homeobox transcription factor family and preferentially expressed in oocytes and germ cells within the mouse ovary. We discovered that Lhx8 knockout females lose oocytes within 7 days after birth. At the time of birth, histological examination shows that Lhx8 deficient (Lhx8(-/-)) ovaries are grossly similar to the newborn wild type ovaries. Lhx8(-/-) ovaries fail to maintain the primordial follicles and the transition from primordial to growing follicles does not occur. Lhx8(-/-) ovaries misexpress oocyte-specific genes such as Gdf9, Pou5f1, and Nobox. Very rapid loss of oocytes may partly be due to drastic the down-regulation of Kit and Kitl in Lhx8(-/-) ovaries. We compared Lhx8(-/-) and wild-type ovaries using Affymetrix 430 2.0 microarray platform. Eighty (44%) of 180 of the genes down-regulated more than 5-fold in Lhx8(-/-) ovaries were preferentially expressed in oocytes, whereas only 3 (2%) of 146 genes up-regulated more than 5-fold in the absence of Lhx8 were preferentially expressed in oocytes. In addition, the comparison of genes regulated in Lhx8(-/-) and Nobox(-/-) newborn ovaries discovered a common set of 34 genes whose expression level is affected in both Lhx8 and Nobox deficient mice. Our findings show that Lhx8 is a critical factor for maintenance and differentiation of the oocyte during early oogenesis and it acts in part by down-regulating the Nobox pathway.
This SuperSeries is composed of the SubSeries listed below.
Overall design: Refer to individual Series
Less...| Accession | PRJNA105729; GEO: GSE11897 |
| Type | Umbrella project |
| Publications | Choi Y et al., "Lim homeobox gene, lhx8, is essential for mouse oocyte differentiation and survival.", Biol Reprod, 2008 Sep;79(3):442-9 |
| Submission | Registration date: 26-Jun-2008
Baylor College of Medicine |
| Relevance | Superseries |
Project Data:
| Resource Name | Number
of Links |
|---|
| Publications |
| PubMed | 1 |
| PMC | 1 |
| Other datasets |
| GEO DataSets | 5 |
GEO Data Details| Parameter | Value |
|---|
| Data volume, Spots | 406237 |
| Data volume, Processed Mbytes | 9 |
| Data volume, Supplementary Mbytes | 48 |
This project encompasses the following 3 sub-projects:
| Project Type | Number of Projects |
| Transcriptome or Gene expression | 3 |
BioProject
accession | Organism | Title |
|---|
| PRJNA109017 | Mus musculus | Transcriptional changes in Lhx8 Null newborn mouse ovaries (Baylor College of Medicine) | | PRJNA109019 | Mus musculus | Microarray Analyses of Newborn Mouse Ovaries Lacking Nobox (Baylor College of Medicine) | | PRJNA109021 | Mus musculus | Ovarian Transcript Expression in Newborn Mouse (Baylor College of Medicine) |
|