BioProject

Casein phosphopeptide-selenium complex (CPP-Se), an organic chelate of casein phosphopeptide and selenium, has been shown to stimulate canine lymphocytes proliferation and cytokines production in our previous research, but its effect on the gene expression of peripheral blood is unclear. More...

Casein phosphopeptide-selenium complex (CPP-Se), an organic chelate of casein phosphopeptide and selenium, has been shown to stimulate canine lymphocytes proliferation and cytokines production in our previous research, but its effect on the gene expression of peripheral blood is unclear. To further investigate the potential mechanism associated with CPP-Se in the process of exerting immunomodulatory function in dogs, our current study used high-throughput sequencing technology to explore the effects of feeding CPP-Se for 30 days on the blood transcriptome of dogs. Compared with control group, a total of 341 differentially expressed genes (DEGs) were identified in CPP-Se group, of which 110 were up-regulated and 231 were down-regulated. Gene ontology (GO) analysis revealed that DEGs were enriched in various biological processes and molecular functions, such as ion transport, transmembrane signaling receptor activity, molecular transducer activity and cytokine activity. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis found that DEGs were mainly implicated in multiple immune-related signaling pathways. Immune-related DEGs were screened by the Immport database and the hub genes of all DEGs or DEGs related to cytokine-cytokine receptor interaction pathway and T cell receptor signaling pathway were identified by Cytoscape. Finally, the reliability of RNA-Seq data was verified by RT-qPCR. Our findings indicated that CPP-Se can change the blood gene expression profile of dogs and the results will serve as a scientific reference for the future development of functional foods to improve pet immunity, as well as a theoretical basis for monitoring and evaluating the immune status of dogs. Overall design: Comparative gene expression profiling analysis of whole blood RNA-seq data for dogs fed without CPP-Se or supplemented with CPP-Se for 30 days. Less...