Specific pathogen-free mice (Mus musculus) (8-week-old, male) were maintained in a biosafety level 2 facility in the Animal Resource Center at the Lille Pasteur Institute for at least two weeks prior to usage to allow appropriate acclimatation.
More...Specific pathogen-free mice (Mus musculus) (8-week-old, male) were maintained in a biosafety level 2 facility in the Animal Resource Center at the Lille Pasteur Institute for at least two weeks prior to usage to allow appropriate acclimatation.
Unless specified, mice were fed a standard rodent chow (SAFE A04) (SAFE, Augy, France) and water ad libitium.
Mice were intranasally (i.n.) infected with PBS containing (or not, in a mock sample) 30 plaque forming units (p.f.u.) of the H3N2 IAV strain A/Scotland/20/1974.
To study the impact of influenza infection on the gut microbiota, caecal samples were collected from different sets of animals including mock-treated mice (D0) and IAV-infected mice D7 and D14.
Caecum homogenates were stored at -80 Celsius until further analysis.
Genomic DNA extraction, shotgun sequencing and microbial community and taxonomic analyses are described in Urien et al. (2023). Less...