BioProject

RNA sequencing of R. toruloides was performed in different contexts. The first ones were stress-related: two conditions of high temperature and two ethanol concentrations, while the other was using the inexpensive and abundant sugarcane juice as substrate. More...

RNA sequencing of R. toruloides was performed in different contexts. The first ones were stress-related: two conditions of high temperature and two ethanol concentrations, while the other was using the inexpensive and abundant sugarcane juice as substrate. Using transcriptomic data, differential expression and functional analysis were implemented to select for differentially expressed genes and enriched pathways from each set-up. A reproducible bioinformatics workflow was developed for mining new regulatory elements. We then predicted, for the first time in this yeast, binding motifs for several transcription factors. Most of the putative transcription factors were involved in stress responses and found in the yeast genome.The novel putative cis-regulatory elements described here offer a great initial point to optimize gene regulation in industrial conditions expanding the current knowledge on regulatory networks for this yeast. Less...