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Accession: PRJNA795486 ID: 795486

RNA degradome analysis reveals substrates of NYN-domain containing DNE1 endoribonuclease in Arabidopsis (thale cress)

See Genome Information for Arabidopsis thaliana
To gain insight into the functional role of DNE1 in cytoplasmic mRNA decay, we performed Parallel Analysis of RNA Ends (PARE) and Global Mapping of Uncapped and Cleaved Transcripts (GMUCT) analysis to identify DNE1-dependent cleavage sites based on their sensitivity to XRN4 within the Arabidopsis transcriptome Overall design: RNA degradome analysis (ATH1036-41; ATH1082-87): Comparing the RNA decay profiles allowed for the detection of abundant monophosphorylated 5' ends (5'P) within transcripts in xrn4 that were absent in dne1xrn4. More...
AccessionPRJNA795486; GEO: GSE193247
Data TypeTranscriptome or Gene expression
ScopeMultiisolate
OrganismArabidopsis thaliana[Taxonomy ID: 3702]
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis; Arabidopsis thaliana
PublicationsNagarajan VK et al., "RNA degradome analysis reveals DNE1 endoribonuclease is required for the turnover of diverse mRNA substrates in Arabidopsis.", Plant Cell, 2023 May 29;35(6):1936-1955
SubmissionRegistration date: 7-Jan-2022
Plant and Soil Sciences, Delaware Biotechnology Institute
NCBI Links
RelevanceModel Organism
Project Data:
Resource NameNumber
of Links
Sequence data
SRA Experiments24
Publications
PubMed1
PMC1
Other datasets
BioSample24
GEO DataSets1
GEO Data Details
ParameterValue
Data volume, Supplementary Mbytes21
SRA Data Details
ParameterValue
Data volume, Gbases71
Data volume, Mbytes29852

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