BioProject

The goal of this study is to find out the exact mechanisms of action of silymarin (SM), a complex mixture of flavonolignans structures isolated from milk thistle (Silybum marianum), on the biological behavior (phenotype) of human HaCaT keratinocytes. More...

The goal of this study is to find out the exact mechanisms of action of silymarin (SM), a complex mixture of flavonolignans structures isolated from milk thistle (Silybum marianum), on the biological behavior (phenotype) of human HaCaT keratinocytes. Overall design: To place the HaCaT cells in a context of a tissue re-epithelization process, we treated these cells for 24 hours with the Transforming Growth Factor β1 (TGF-β1, 10 ng/ml) in presence or absence of silymarin (SM, 31.25 µg/ml). Total RNA (longer than 200 nucleotides) were isolated, polyA RNA were enriched and sequenced from 3 biological replicate samples of TGF-β1-treated HaCaT cells (TGF) and 3 biological replicate samples of TGF-β1 plus SM-treated HaCaT cells (TGF.SM31). Less...