BioProject

Macrocylic lactones, milbemectin and abamectin, are compounds that are frequently used for the control of the two-spotted spider mite Tetranychus urticae. More...

Macrocylic lactones, milbemectin and abamectin, are compounds that are frequently used for the control of the two-spotted spider mite Tetranychus urticae. The development of abamectin resistance in this major pest has become an increasing problem worldwide, potentially compromising the use of milbemectin. In this study, gene expression profile differences between abamectin/milbemectin resistant T. urticae populations (ES1, IT2 and UK6) and a susceptible T. urticae population (RO1 and IT3) were compared using Illumina RNAseq. Overall design: In total, 20 RNA-seq samples (5 populations, 4 biological replicates per population) from Tetranychus urticae were generated. RNA was collected from adult T. urticae females. For each sample, Illumina mRNA-seq libraries were constructed and stranded, paired-end 125 bp (RO1, IT2, IT3 and ES1) or 150 bp (UK6) RNA-seq Illumina reads were generated. The obtained reads were used to quantify gene expression in each sample. Less...