BioProject

Glioblastoma multiforme (GBM) is a highly aggressive and vascularized malignant brain tumor. More...

Glioblastoma multiforme (GBM) is a highly aggressive and vascularized malignant brain tumor. SoxF transcription factors consisting of Sox7, Sox17, and Sox18 are expressed specifically in endothelial cells (ECs) and contribute to vascular morphogenesis. While the role of Sox17 was found in subcutaneous ectopic tumors, Sox7 has not been studied in the context of tumor angiogenesis. Here, we investigated gene expression profile of RNA analysis of Sox7- and Sox17-deficient mouse endothelial cells from high grade glioma using RNA sequencing to validate molecular characteristics of Sox7 and Sox17 in high grade glioma. Overall design: To reveal molecular characteristics of Sox7 and Sox17 in high-grade glioma endothelial cells, we established mouse orthotopic glioma model by intracranially injecting GL26l glioma cells into congenic B6 genetic background mice. To excise Sox7 and Sox17 in ECs, the Cdh5(BAC)-CreERT2 driver line was bred with Sox7fl/fl and Sox17fl/fl mice and experimental mice intraperitoneally administered 20 mg/kg tamoxifen. For in-depth understanding of different gene expression of Sox7- and Sox17- deficient vessels, we purified endothelial population and performed RNA-sequencing analysis. There are two independent sets of Sox7-deficient and control group, and three independent sets of Sox17-deficient and control group. Less...