BioProject

Synergists can counteract metabolic insecticide resistance by inhibiting detoxification enzymes or transporters. More...

Synergists can counteract metabolic insecticide resistance by inhibiting detoxification enzymes or transporters. In this study we used Illumina RNA-sequencing to investigate genome-wide transcriptional responses in an acaricide resistant strain (JP-R) of the spider mite Tetranychus urticae upon exposure to synergists such as S,S,S-tributyl phosphorotrithioate (DEF), diethyl maleate (DEM), piperonyl butoxide (PBO) and cyclosporin A (CsA). Overall design: In total 24 RNA-seq samples (6 treatments, 4 biological replicates per treatment) from Tetranychus urticae, were generated. RNA was collected from adult T. urticae females 24h after being sprayed with a synergist compound (PBO, DEF, DEM or CsA) or with formulation only (FORM). Unsprayed mites served as an additional control (CON). For each sample, Illumina mRNA-seq libraries were constructed and strand-specific paired reads of 2x125 bp were generated. The obtained reads were used to quantify gene expression in each sample. Less...