BioProject

Lung fibroblasts play an important role in extracellular matrix homeostasis and this process is mainly regulated by transforming growth factor-beta (TGF-β). More...

Lung fibroblasts play an important role in extracellular matrix homeostasis and this process is mainly regulated by transforming growth factor-beta (TGF-β). Hence, lung fibroblasts are postulated to play a crucial role in aberrant lung tissue repair and remodeling, which is a main factor in lung diseases like COPD. In this study, the effect of TGF-β1 on the miRNA expression in parenchymal lung fibroblasts is investigated. Overall design: Primary parenchymal lung fibroblasts of 9 control subjects were cultured. After serum starving for 24 hrs, the fibroblasts were stimulated with and without TGF-β1 (100 U/ml TGF-β1, equivalent to 3.1 ng/ml) for 24 hours. The RNA was isolated using TRIzol according to the protocol of the manufacturer. The RNA samples were then hybridized with the Human GeneChip miRNA 1.0 array (Affymetrix). Less...