In the murine system, Oct4, Sox2, c-Myc and Klf4 are sufficient to convert fibroblasts to induced pluripotent stem (iPS) cells that exhibit many characteristics of embryonic stem (ES) cells. Herein, we show that the orphan nuclear receptor Esrrb works in conjunction with Oct4 and Sox2 to mediate reprogramming of mouse embryonic fibroblasts (MEFs) to iPS cells. Esrrb reprogrammed cells share similar expression and epigenetic signatures as ES cells. These cells are also pluripotent and can differentiate in vitro and in vivo into the three major embryonic cell lineages. Furthermore, these cells contribute to mouse chimeras and are germline transmissible. In ES cells, Esrrb targets many genes involved in selfrenewal and pluripotency. This suggests that Esrrb may mediate reprogramming through the up-regulation of ES cell-specific genes.
More...In the murine system, Oct4, Sox2, c-Myc and Klf4 are sufficient to convert fibroblasts to induced pluripotent stem (iPS) cells that exhibit many characteristics of embryonic stem (ES) cells. Herein, we show that the orphan nuclear receptor Esrrb works in conjunction with Oct4 and Sox2 to mediate reprogramming of mouse embryonic fibroblasts (MEFs) to iPS cells. Esrrb reprogrammed cells share similar expression and epigenetic signatures as ES cells. These cells are also pluripotent and can differentiate in vitro and in vivo into the three major embryonic cell lineages. Furthermore, these cells contribute to mouse chimeras and are germline transmissible. In ES cells, Esrrb targets many genes involved in selfrenewal and pluripotency. This suggests that Esrrb may mediate reprogramming through the up-regulation of ES cell-specific genes. Our findings also indicate that it is possible to reprogram MEFs without exogenous Klf transcription factors and link a nuclear receptor to somatic cell reprogramming.
This SuperSeries is composed of the SubSeries listed below.
Overall design: Refer to individual Series
Less...Accession | PRJNA109479; GEO: GSE13190 |
Type | Umbrella project |
Publications | Feng B et al., "Reprogramming of fibroblasts into induced pluripotent stem cells with orphan nuclear receptor Esrrb.", Nat Cell Biol, 2009 Feb;11(2):197-203 |
Submission | Registration date: 15-Jan-2009 Genome Institute of Singapore |
Relevance | Superseries |
Project Data:
Resource Name | Number of Links |
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Publications |
PubMed | 1 |
Other datasets |
GEO DataSets | 3 |
GEO Data DetailsParameter | Value |
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Data volume, Spots | 1585488 |
Data volume, Processed Mbytes | 45 |
Data volume, Supplementary Mbytes | 59 |
This project encompasses the following 2 sub-projects:
Project Type | Number of Projects |
Transcriptome or Gene expression | 2 |
BioProject accession | Organism | Title |
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PRJNA114035 | Mus musculus | Global gene-expression analyses of the Esrrb reprogrammed cells (Genome Institute of Singapore) | PRJNA114037 | Mus musculus | Global gene-expression analyses of the Esrrb knockdown cells (Genome Institute of Singapore) |
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