BioProject

Soil cores were collected from the rhizosphere of 81 temperate trees planted in a temperate forest restoration project. More...

Soil cores were collected from the rhizosphere of 81 temperate trees planted in a temperate forest restoration project. Species included Tulip tree (Liriodendron tulipifera), Black Cherry (Prunus serotina), and Red Oak (Quercus rubra). DNA was extracted from rhizosphere soils using a phenol-chloroform protocol. Amplicons of the fungal ITS-2 region were made with the primers 58A2F and NLB4 containing Illumina overhang adapters. Amplicons were purified, indexed, and sequenced as 2 x 250bp reads on one lane of the Illumina MiSeq V3 sequencer at the Case Western Reserve University Genomics Core. Less...