Secretory dysfunction of salivary glands (SG) from Sjogren syndrome (SS)-patients has been associated with altered homeostasis, upregulation of the ATF6a transcription factor, which participates in the UPR and ERAD components, such as SEL1L.
More...Secretory dysfunction of salivary glands (SG) from Sjogren syndrome (SS)-patients has been associated with altered homeostasis, upregulation of the ATF6a transcription factor, which participates in the UPR and ERAD components, such as SEL1L. In three dimensional (3D)-acini, ATF6a and SEL1L mRNA levels increased in response to IFN-g, but the mechanism involved is unknown. Here, we characterized the global profile of small RNAs (sRNAs) in SG from SS-patients and controls with functional emphasis on hsa-miR-424-5p, which is predicted to target ATF6a and SEL1L mRNAs.
sRNA next generation sequencing (NGS) was employed to identify miRNAs differentially expressed between SG from SS-patients and controls. Taqman assays validated hsa-miR-424-5p levels in SG from 16 SS-patients and 8 controls and these values were correlated with ATF6a and SEL1L mRNA levels. Functional assays involved IFN-g stimulation 3D-acini and chemically-synthesized hsa-miR-424-5p mimics and inhibitors. Less...