BioProject

CHO cells have primarily been studied in bulk, assuming that these bulk samples are identical because of genetic clonality across the sample. More...

CHO cells have primarily been studied in bulk, assuming that these bulk samples are identical because of genetic clonality across the sample. In this study, we performed single-cell RNA sequencing on two clonal CHO-K1 cell populations with different stability phenotypes over a 90 day culture period. Overall design: Two Chinese Hamster Ovary were cultured for 30 population doublings in different culture conditions, with samples gathered before the first and final passages. Banked samples were sequenced with scRNA sequencing. --------------------------------------------- Authors state "due to company restrictions (our sample source) they do not want us to deposit the raw FASTQ (Level 1) but will allow the counts matrix to be deposited". Less...