BioProject

Microbiome analysis of oropharyngeal and nasopharyngeal samples of SARS-CoV-2 infected patients Overall design: Analysis of the the different microbial species detected in the oropharyngeal and nasopharyngeal samples of the SARS-CoV-2 patients We have developed this technology as a potential resource that can be used as a sensitive diagnostic tool for early detection of highly infectious microorganisms in clinical samples, which includes all known respiratory viruses as well as SARS-CoV-2. More...

Microbiome analysis of oropharyngeal and nasopharyngeal samples of SARS-CoV-2 infected patients Overall design: Analysis of the the different microbial species detected in the oropharyngeal and nasopharyngeal samples of the SARS-CoV-2 patients We have developed this technology as a potential resource that can be used as a sensitive diagnostic tool for early detection of highly infectious microorganisms in clinical samples, which includes all known respiratory viruses as well as SARS-CoV-2. DNA and RNA were isolated from nasopharyngeal (NP) and oropharyngeal swabs (OP), which were then subjected to whole transcriptome amplification (WTA) where the amplified products were used to hybridize against probes on specifically designed PathoChIPs containing sequences that are unique, conserved and frequently mutated across various regions of the SARS-CoV-2 genome. This PathoChip (V5) contains 60,000 probes and represents all known viruses, including SARS-CoV-2, and pathogenic microbes that comprise 250 helminths, 130 protozoa, 360 fungi, and 320 bacteria, totaling more than 6,000 accessions. Less...