See
Genome Information for Mus musculus
We performed differential gene expression analysis using high throughput multiplex analysis via NanoString nCounter PanCancer Immune Profiling panel, which analyzes 770 genes related to cancer-immune pathways (cancer progression, chemokines and cytokines and their receptors, and innate and adaptive immune response). nSolver software was used to analyze the data and a heat map was generated to show differential expression of 770 genes. Further, using a fold cut-off of ≥2 and p-value <0.05, we observed that 4'-BR significantly modulated expression of multiple cancer immune genes.
Overall design: Tumors were induced by topical application of 4-hydroxy-tamoxifen on shaved backs of 10-week-old mice, and the effects of 4′‐BR (30 mg/kg b.wt.; intraperitoneally; 3d/week for 5 weeks) were assessed on melanoma development and progression. RNA was extracted from tumors obtained from control and 30 mg/kg 4′‐BR treated mice (n = 6), followed by determination of expression of 770 genes (730 immune genes and 40 housekeeping genes) via the Mouse PanCancer Immune Profiling Panel using the nCounter platform in the NanoString Technologies .
| Accession | PRJNA708302; GEO: GSE168564 |
| Data Type | Transcriptome or Gene expression |
| Scope | Multiisolate |
| Organism | Mus musculus[Taxonomy ID: 10090] Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus; Mus musculus |
| Publications | Chhabra G et al., "Antimelanoma Effects of Concomitant Inhibition of SIRT1 and SIRT3 in Braf(V600E)/Pten(NULL) Mice.", J Invest Dermatol, 2022 Apr;142(4):1145-1157.e7 |
| Submission | Registration date: 9-Mar-2021
Ahmad Lab, Dermatology, University of Wisconsin |
| Relevance | Model Organism |
Project Data:
| Resource Name | Number
of Links |
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| Publications |
| PubMed | 1 |
| PMC | 1 |
| Other datasets |
| GEO DataSets | 1 |
GEO Data Details| Parameter | Value |
|---|
| Data volume, Spots | 4620 |
| Data volume, Processed Mbytes | 1 |
| Data volume, Supplementary Mbytes | 1 |