Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
Organizing biological data
VEB-9 positive XDR-CRPA isolates Pae51 and Pae52
Transcriptional profiling of CD11b+ microglia isolated from cerebellum and olfactory bulb-removed brains of SAMP8 and SAMR1 mice at 2, 5, and 9 months of age.
Bacteria
Genome sequencing of mcr-9 positive strains
Custom 9-Gene Targeted Next-Generation Sequencing Panel for Thyroid Fine Needle Aspirations in China
Genome sequencing of mcr-9-carrying Gram-negative bacteria
CA19-9-positive extracellular vesicles are the risk for cancer-associated thrombosis in pancreatic cancer
Rice paddy soil microbiome 16S rRNA: Household biomass ash as a fertilizer to sustainability
16S-soil-ash-Bangladesh, Here we investigated in large scale field trials across two geographic regions and two seasons, if domestic hearth ash can be used as a fertilizer for paddy rice cultivation, how geographic, season and application of household ash to soil impact on rice yield, and grain quality (accumulation of As, Cd, alongside nutrient elements) and rhizosphere soil microbial composition. The study was conducted in 9 homesteads each for two geographic regions (Madhupur & Barind tract), over two seasons (wet &dry). In each season, the following 3 treatments were applied: 1=control (conventional NKPS), 2= Ash (ash to supply all required P plus urea and K), 3 =control plus ash (1 t ash per ha plus conventional NPKS). At the end of each season, grain yield, mineral elements in grain (ICP-MS) and rhizosphere soil microbial community (16S rRNA amplicon sequencing, Illumina) were assessed and at the end of the overall experiment the overall impact on mineral elements in soil (XRF) evaluated.
mcr-9/10-positive Enterobacter cloacae complex strains
mcr-9 positive Salmonella enterica
Dissecting iNKT cell selection and subset differentiation in real-time
To systematically investigate the earliest phases of iNKT cell development and subsequent subset differentiation, we generated a genetic system to induce a timed wave of iNKT cell generation upon 4-hydroxytamoxifen (4-OHT) administration in mice bearing CD4-CreERt2 (Sledzinska et al., 2013) and Vα14iStopF (Vahl et al., 2013) knock-in transgenes. We globally investigated the transcriptional changes that guide early iNKT cell development by bulk 3’-sequencing of poly(A)-RNA of induced developing iNKT cells between 12h and 5d after induction in comparison to DP thymocytes, stage 0 CD24+ iNKT cells as well as NKT1, NKT2 and NKT17 mature subsets. Briefly, CD4-CreERt2 Vα14iStopF (± Traj18KO) mice were administered with 4-OHT and sacrificed at different timepoints after administration (between 12h and 120h). 1000 cells of the following samples were FACS purified for RNA-seq analyses: 9 timepoints of induced iNKT cells (from 12h to 120h) (gated as mCD1d-PBS57- Tetramer+ CD44low NK1.1−); DP = DP thymocytes (TCRβ− mCD1d-PBS57-Tetramer− CD4+ CD8+ CD69−); CD24+ = early wildtype iNKT cells (mCD1d-PBS57-Tetramer+ CD44− CD24+); NKT1 (mCD1d-PBS57-Tetramer+ CD44+ NK1.1+ CD27+ CD138−); NKT2 (mCD1d- PBS57-Tetramer+, NK1.1− PLZFeGFP+ ICOS+ IL17RB+ CD138−); NKT17 (mCD1d- PBS57-Tetramer+ CD19− ICOS+ CD138+). For each condition, four biological replicates were sequenced (except for 96h timepoint, where two samples of the same mouse where sequenced). For the NKT17 samples and two NKT2 samples, 2 mice were pooled. For CD24+ samples, 4-6 mice were pooled. Library preparation for bulk 3’-sequencing of poly(A)-RNA was done as described previously (Parekh et al., 2016). The library was sequenced on a NextSeq 500 (Illumina) with 16 cycles for the barcodes and UMIs and 50 cycles for the cDNA.
Pharmacological inhibition of CDK4/6 augments long-term anti-tumor immunity through the induction of T cell memory [9_RNAseq_in_vitro_human_LeY_CAR]
Escherichia coli
Whole genome sequence of mcr-9 positive Escherichia coli derived from pigs.
Exome-Seq of murine MB49 cell line - WT and gal-9-KO - in vitro
Exome-Seq of murine CT26 cell line - WT and gal-9-KO - in vitro
Elucidating The Role Of ∆9-Tetrahydrocannabinol (Thc) And Cannabidiol (Cbd) In Developmental Toxicity
mRNA transcriptional profiles in Streptococcus thermophilus wild-type strain LMD-9 and 3 mutants deleted for the pptAB, eep and amiCDE genes.
RNAseq of murine MB49 cell line - WT and gal-9-KO - in vitro
RNAseq of murine CT26 cell line -WT and gal-9-KO - in vitro
RNAseq of murine MB49 tumors - WT and gal-9-KO - grown on C57BL/6 syngenic mice through serial transplantations
ISX-9 manipulates endocrine progenitor fate revealing conserved intestinal lineages in mouse and human
Filters: Manage Filters
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on