BioProject

This study compares the dose dependent transcriptome data obtained for the volatile compound dimethylamine, which has been exposed in an in vitro system via air-liquid-interface (ALI) exposure to a pulmonary cell line (A549 cells). More...

This study compares the dose dependent transcriptome data obtained for the volatile compound dimethylamine, which has been exposed in an in vitro system via air-liquid-interface (ALI) exposure to a pulmonary cell line (A549 cells). Overall design: A549 cells were exposed to dimethylamine under ALI-conditions in 12-well plates (P.R.I.T.® ExpoCube®, Ritter et al. 2018 ). Exposure took place for 60 minutes by applying exposure flows of 3 ml/min per 1cm² ALI culture. The experimental design was based on 3 groups including 4 cultures / plate being exposed to the test substance, 4 cultures / plate being exposed to clean air as exposure control and the remaining wells / plate as non-exposure controls (no application of exposure flow). Vapor concentrations were set up by controlled in-line evaporation of the liquids using an impinger and dilution with clean air. Concentrations were monitored online using quantitative FT-IR analysis (Gasmet DX4000). High dose (HD) = 81.7 ppm, mid dose (MD) = 41.1 ppm, low dose (LD) = 14.9 ppm. Exposure for one hour was followed by a 23hr recovery period before the next exposure started. Within a 72hr interval a total of 3 exposure and recovery periods were accomplished without medium change in between. For each exposure condition 3 biological replicates where tested, each was based on 2 pooled wells. Less...