BioProject

To clarify the optic nerve head (ONH) gene expression responses associated with a single, axon-damaging exposure to elevated IOP in relation to the composite cellular events previously identified in chronically elevated IOP glaucoma models. More...

To clarify the optic nerve head (ONH) gene expression responses associated with a single, axon-damaging exposure to elevated IOP in relation to the composite cellular events previously identified in chronically elevated IOP glaucoma models. Anesthetized rats were exposed unilaterally to an 8-hour pulse-train controlled elevation of IOP (PT-CEI) at 60 mm Hg, while others received normotensive CEI at 20 mmHg. ONH RNA was harvested at 0 hr, and 1, 2, 3, 7, and 10 days after PT-CEI and normotensive-CEI, in addition to naive animals. RNA-Seq was performed to analyze ONH gene expression. The number of significantly changed genes peaked immediately (n of 1354) after the PT-CEI (0 hr). This was followed by a lull (less than 4 genes per time point) at 1 and 2 days post PT-CEI. Gene activity increased again at 3 days (136 genes) and persisted at 7 (78 genes) and 10 (339 genes) days. The PT-CEI model places in sequence ONH gene expression responses previously reported in models with chronically elevated IOP and may provide insights into their role in optic nerve damage. Less...