In our previous study, quorum quenching (QQ) bacteria can effectively enhanced methane production in the process of mitigate anaerobic membrane bioreactor (AnMBR) membrane biofouling. However, the mechanisms on how the QQ bacteria affected anaerobic system is unclear. In this study, the effects of QQ bacteria on the processes of hydrolysis, acidogenesis, acetogenesis and methanogenesis were studied in detail. The cumulative methane production improved by 26.13%, 22.54%, 48.70% and 44.93% at QQ bacteria dosage of 0.5, 1, 5 and 10 mg strain/g beads, respectively. It was found that the presence of QQ bacteria enhanced acidogenesis step resulting in higher VFA production, while it had no obvious influence on hydrolysis, acetogenesis and methanogenesis steps. The substrate (glucose) conversion efficiency in acidogenesis step was also accelerated (QQ was 1.45 folds than control within first eight hours). The abundance of hydrolytic fermentation gram-positive bacteria and several acidogenic bacteria, such as Hungateiclostridiaceae, was promoted in QQ amended culture, which enhanced VFA production and accumulation. Although the abundance of acetoclastic methanogen Methanosaeta reduced by 54.2% on the 1st day of QQ beads addition, the overall performance of methane production was not affected. This study revealed that QQ had a greater impact on the acidogenesis step in the anaerobic digestion process, though the microbial community in acetogenesis and methanogenesis steps was altered.
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