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Accession: PRJNA925880 ID: 925880

Human immunodeficiency virus 1

HIV-PULSE: A long-read sequencing assay for high-throughput near full-length HIV-1 proviral genome characterization

See Genome Information for Human immunodeficiency virus 1
The HIV-PULSE (HIV Proviral UMI-mediated Long-read Sequencing) assay uses unique molecular identifiers (UMIs) to tag individual HIV-1 genomes, allowing for the omission of the limiting dilution step and enabling long-range PCR amplification of many NFL genomes in a single PCR reaction, while simultaneously overcoming poor single-read accuracy.
AccessionPRJNA925880
Data TypeGenome sequencing and assembly
ScopeMultiisolate
OrganismHuman immunodeficiency virus 1[Taxonomy ID: 11676]
Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus; Human immunodeficiency virus 1
Publications
  • Published online: Lambrechts L et al., "HIV-PULSE: A long-read sequencing assay for high-throughput near full-length HIV-1 proviral genome characterization", bioRxiv, 2023;
SubmissionRegistration date: 20-Jan-2023
Ghent University
RelevanceMedical
Locus Tag PrefixPJX97
Project Data:
Resource NameNumber
of Links
Sequence data
SRA Experiments35
Other datasets
BioSample3
SRA Data Details
ParameterValue
Data volume, Gbases55
Data volume, Mbytes47482

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