Our study compares the nasopharyngeal microbiome of the deceased RSV+ and deceased RSV- infants from the Zambia Pertussis and RSV Infant Mortality Estimation (ZPRIME) study. Infants were between four days and six months old and enrolled in the ZPRIME study within 48 hours of death. We selected a subset of community deaths for our analysis who were RSV+ with a PCR Ct<35. We selected 50 RSV+ infants via random sampling from and matched those to 50 RSV- infants from the same study. Due to age- and seasonality-related variations in RSV impact, we matched infants by age at death (4-28 days (0+ months), 29-61 days (1+ month), 62-152 days (2+ months) and 152-182 days (4+ months)) and date of death (+/- 1.5 months). All decedents underwent one-time, post-mortem NP sampling. NP samples were tested for RSV by reverse transcriptase quantitative PCR following the testing protocol developed by the Centers for Disease Control and Surveillance. 16S ribosomal DNA was amplified using PCR with primers specific to the V3-V4 region.
| Accession | PRJNA913857 |
| Data Type | Raw sequence reads |
| Scope | Multispecies |
| Grants | - "Removing batch effects in genomic and epigenomic studies" (Grant ID R01 GM127430, National Institute of General Medical Sciences)
- "Signature of profiling and staging the progression of TB from infection to disease." (Grant ID R21 AI154387, National Institute of Allergy and Infectious Diseases Extramural Activities)
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| Submission | Registration date: 19-Dec-2022
Rutgers, The State University of New Jersey |
| Relevance | Medical |
Project Data:
| Resource Name | Number
of Links |
|---|
| Sequence data |
| SRA Experiments | 100 |
| Other datasets |
| BioSample | 100 |