BioProject

9 days after germination, 35S::ESR2-ER (Ikeda et al, 2007; Eklund et al, 2011) seedlings were induced with beta-estradiol and shoot tissue was colected 30 minutes and 8 hours after induction. More...

9 days after germination, 35S::ESR2-ER (Ikeda et al, 2007; Eklund et al, 2011) seedlings were induced with beta-estradiol and shoot tissue was colected 30 minutes and 8 hours after induction. 30 minutes treatments: 10 uM beta-estradiol plus 30 uM cycloheximide dissolved in DMSO (induction), 30 uM cycloheximide dissolved in DMSO (cycloheximide control) or DMSO (same volume as beta-estradiol treatment; mock treatment). 8 hours treatments: 10 uM beta-estradiol dissolved in DMSO (induction) or DMSO (same volume; mock). RNA-Seq libraries were prepared using the TruSeq Stranded mRNA Sample Preparation Guide, Part #15031047 Rev. E. Sequencing was done on an Illumina HiSeq, 100 bases paired-end. Less...