Supplementary Table 1 Primers of RT-PCR
Gene Primer sequence (5’-3’)
carA Forward GACAGCGGCGTGGTGATGATC
Reverse CTTGTGAGTGGCTCGCAGGTTG
carB Forward CGTGCCGTTCGTCTCCAAGTG
Reverse GCGGGATGATTTCCTGGGTGAAG
pyrB Forward ACCGTGGCGTGGAGATCGAG
Reverse GCTGGCGTTGGGTGTTCTGG
pyrC Forward AACTCGGACTCTGGCGTGACC
Reverse GTGCTCGTCGATGAACTGCTTCTC
pyrE Forward GCGGTGATCGACAGCGGAATC
Reverse GTGGTCCTTGGCTTCCTTGCG
pyrF Forward CGACCTGAAATTCCACGACATCCC
Reverse GTTGACCATCCACACGCCCATC
luxS Forward AAGTCCTATGGGTTGCCAAACTGG
Reverse GCAGGCACTTCAGTAGCATTTAGC
saeR Forward CGCCTTAACTTTAGGTGCAGATGAC
Reverse ACGCATAGGGACTTCGTGACCATT
sigB Forward GGTGCCATAAATAGATTCGATATGTCCTT
Reverse CTTTTGATTTCACCGATTACAGTAGGTACT
cidA Forward AGCGTAATTTCGGAAGCAACATCCA
Reverse CCCTTAGCCGGCAGTATTGTTGGTC
icaA Forward GTTGTCGACGTTGGCTACTG
Reverse ATGGCAAGCGGTTCATACTT
atlA Forward AACAGCACCAACGGATTAC
Reverse CATAGTCAGCATAGTTATTCATTG
psmα Forward GGCCATTCACATGGAATTCGTAG
Reverse TAGCCATCGTTTTGTCCTCCTG
psmβ Forward GGACTAGCAGAAGCAATCGCA
Reverse CCTAGTAAACCCACACCGTTAGC
gyrB Forward GGAGGTAAATTCGGAGGT
Reverse CTTGATGATAAATCGTGCCA
Supplementary Table 2 Sequence of antisense oligonucleotides for LuxS
Oligonucleotides Sequence (5’-3’)
Oligo 1 T*A*G*C*G*G*G*A*A*C*G*A*T*G*G*A*A*G*G*A
Oligo 2 T*A*T*G*G*A*T*A*T*G*C*C*C*G*G*A*T*T*G*C
Oligo 3 G*G*G*T*T*G*C*C*A*A*A*C*T*G*G*T*T*T*C*T
Accession | PRJEB54101 |
Scope | Monoisolate |
Submission | Registration date: 7-Jul-2022 the fourth military medical university |
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