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Accession: PRJEB54101 ID: 856587

Pyrancoumarin Derivative LP4C Targeting of Pyrimidine de novo Synthesis Pathway Inhibits MRSA Biofilm and Virulence

Sequence of antisense oligonucleotides for LuxS and the sequence of related primers

Supplementary Table 1 Primers of RT-PCR Gene Primer sequence (5’-3’) carA Forward GACAGCGGCGTGGTGATGATC Reverse CTTGTGAGTGGCTCGCAGGTTG carB Forward CGTGCCGTTCGTCTCCAAGTG Reverse GCGGGATGATTTCCTGGGTGAAG pyrB Forward ACCGTGGCGTGGAGATCGAG Reverse GCTGGCGTTGGGTGTTCTGG pyrC Forward AACTCGGACTCTGGCGTGACC Reverse GTGCTCGTCGATGAACTGCTTCTC pyrE Forward GCGGTGATCGACAGCGGAATC Reverse GTGGTCCTTGGCTTCCTTGCG pyrF Forward CGACCTGAAATTCCACGACATCCC Reverse GTTGACCATCCACACGCCCATC luxS Forward AAGTCCTATGGGTTGCCAAACTGG Reverse GCAGGCACTTCAGTAGCATTTAGC saeR Forward CGCCTTAACTTTAGGTGCAGATGAC Reverse ACGCATAGGGACTTCGTGACCATT sigB Forward GGTGCCATAAATAGATTCGATATGTCCTT Reverse CTTTTGATTTCACCGATTACAGTAGGTACT cidA Forward AGCGTAATTTCGGAAGCAACATCCA Reverse CCCTTAGCCGGCAGTATTGTTGGTC icaA Forward GTTGTCGACGTTGGCTACTG Reverse ATGGCAAGCGGTTCATACTT atlA Forward AACAGCACCAACGGATTAC Reverse CATAGTCAGCATAGTTATTCATTG psmα Forward GGCCATTCACATGGAATTCGTAG Reverse TAGCCATCGTTTTGTCCTCCTG psmβ Forward GGACTAGCAGAAGCAATCGCA Reverse CCTAGTAAACCCACACCGTTAGC gyrB Forward GGAGGTAAATTCGGAGGT Reverse CTTGATGATAAATCGTGCCA Supplementary Table 2 Sequence of antisense oligonucleotides for LuxS Oligonucleotides Sequence (5’-3’) Oligo 1 T*A*G*C*G*G*G*A*A*C*G*A*T*G*G*A*A*G*G*A Oligo 2 T*A*T*G*G*A*T*A*T*G*C*C*C*G*G*A*T*T*G*C Oligo 3 G*G*G*T*T*G*C*C*A*A*A*C*T*G*G*T*T*T*C*T
AccessionPRJEB54101
ScopeMonoisolate
SubmissionRegistration date: 7-Jul-2022
the fourth military medical university
Project Data:
No public data is linked to this project. Any recently released data that cites this project will be linked to it within a few days.

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