See
Genome Information for Arabidopsis thaliana
ChIP-Seq were performed using 35S::GFP-ASF1A, pHTR13::HTR13-HA, pHTR5::HTR5-HA, and pHTR5::HTR5-HA/pif7-1 transgenic plants after white light and 1 hr shade treatment. Two biological replicates were prepared for each genotype of plants grown under light and shade conditions. High-confidence binding peaks with cut off fold change > 1.5 and P-value <0.01. Our data reveal that ASF1 directly regulating a subset of target genes of PIF7. Furthermore, shade-elicited gene activation is accompanied by H3.3 enrichment, which is mediated by the PIF7-ASF1 regulatory module.
Overall design: ChIP-seq was applied to comprehensively profile the ASF1A/H3.3/H3.1 landscape in response to shade exposure. 8-day-white-light grown seedlings were treated 1 hr shade before harvesting for pHTR13::HTR13-HA, pHTR5::HTR5-HA, pHTR5::HTR5-HA/pif7-1 DNA extraction using HA antibodies. 6-day-white-light grown seedlings were treated 1 hr shade before harvesting for 35S::GFP-ASF1A DNA extraction using GFP antibodies.
| Accession | PRJNA798605; GEO: GSE193955 |
| Data Type | Epigenomics |
| Scope | Multiisolate |
| Organism | Arabidopsis thaliana[Taxonomy ID: 3702] Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis; Arabidopsis thaliana |
| Submission | Registration date: 19-Jan-2022
school of life sciences, Fudan university |
| NCBI Links | |
| Relevance | Model Organism |
Project Data:
| Resource Name | Number
of Links |
|---|
| Sequence data |
| SRA Experiments | 40 |
| Other datasets |
| BioSample | 40 |
| GEO DataSets | 1 |