BioProject

The goal of the RNA sequencing is to find out the signal pathway the VQ28 gene involved in. Uninfected 30-day-old detached Arabidopsis rosette leaves of wild type Col-0, knock-out line VQ28KO12 and overexpression line VQ28OE9 were collected to isolate total RNA. Then, we created a final cDNA library and performed the 2×150bp paired-end sequencing on an Illumina Novaseq™ 6000. After removed the low quality bases and undetermined bases, we mapped reads to the genome. The differentially expressed mRNAs were selected with fold change > 2 or fold change < 0.5 and p value < 0.05, and then analysis GO enrichment and KEGG enrichment to the differentially expressed mRNAs. In comparison with Col-0, constitutively overexpressing VQ28 caused the enrichment of most genes in MAPK signaling pathway and plant hormone signal transduction. Among the 11 genes up-regulated in VQ28OE9 but down-regulated in VQ28KO12, examining their expression by querying the Arabidopsis eFP Browser which contains an extensive collection of gene expression microarray data, six of them were involved in the response to abscisic acid (ABA). qRT-PCR analyses of expression presented that the six ABA-related genes were up-regulated in VQ28OE9 without infection. Overall design: Examination of mRNA level in Col-0,VQ28KO12 and VQ28OE9 detached leaves