BioProject

Seeds were planted in a Percival growth chamber (Percival model E-41L2) with standard potting mix (40% Canadian peat, 40% coarse vermiculite, 15% masonry sand, and 5% screened topsoil) and target conditions of 111 mol m(-2) s(-1) light intensity, 60% relative humidity, a 12 h/12 h day night cycle with a target temperature of 29 C during the day and 23 C at night. More...

Seeds were planted in a Percival growth chamber (Percival model E-41L2) with standard potting mix (40% Canadian peat, 40% coarse vermiculite, 15% masonry sand, and 5% screened topsoil) and target conditions of 111 mol m(-2) s(-1) light intensity, 60% relative humidity, a 12 h/12 h day night cycle with a target temperature of 29 C during the day and 23 C at night. In order to target approximately the three leaf stage for different species, planting dates were staggered to allow simultaneous cold stress treatment for batches of seedlings from multiple species: foxtail millet (genotype Yugu1), pearl millet (genotype USDA PI 583800), switchgrass (genotype kanlow), and proso millet (genotype earlybird/USDA PI 578073) seedlings of 12,10, 17, and 14 days after planting were used. For cold stress treatment, seedlings reaching the desired growth stage described above were divided with one half of each variety transferred to a growth chamber maintained at a consistent 6 C and with other settings equivalent to the control samples. The transfer to cold stress treatment was always timed to occur at the end of the 12hour day cycle. Paired samples were collected from control and cold stress treatments at 0.5, 1, 3, 6, 16, and 24 h after the onset of cold stress. Each sample was a pool of all above ground tissue from at least three individual seedlings. Samples were collected from a total of three independent biological replicates grown and harvested at separate dates (1 replicate of control for pearl millet at 16h was eliminated due to low sequence quality). Less...