BioProject

Globally, the three categories of intact SWI/SNF complexes in control cells have shared and unique binding peaks, with cBAF showing the most abundant binding sites followed by ncBAF and pBAF. More...

Globally, the three categories of intact SWI/SNF complexes in control cells have shared and unique binding peaks, with cBAF showing the most abundant binding sites followed by ncBAF and pBAF. In control cells, within cBAF, ARID1B bound to only a subset of ARID1A peaks. In control cells, the binding pattern of BAF45d corresponded to ARID1A patterns, but binding was virtually eliminated in ARID1-less cells. In ARID1-less cells, ARID2 binding was essentially eliminated whereas Brd9 binding was unaltered. Overall design: ChIP-Seq of SWI/SNF subunits in WT and ARID1-less H2.35 cells. The ChIPs were all performed using a Ty1 antibody (https://www.diagenode.com/en/p/ty1-monoclonal-antibody-classic-50-ug-25-ul). Different proteins indicated in each file name were cloned with a 3xTy1 tag at the C-terminal and expressed in WT and ARID1-less (ARID1A inducible-KD and ARID1B KO) H2.35 cells, followed by ChIP using Ty1 antibody. Less...